This ready reference presents environmentally friendly and stereoselective methods of modern biocatalysis. The experienced and renowned team of editors have gathered top international authors for this book. They cover such emerging topics as chemoenzymatic methods and multistep enzymatic reactions, while showing how these novel methods and concepts can be used for practical applications. Multidisciplinary topics, including directed evolution, dynamic kinetic resolution, and continuous-flow methodology are also discussed.
From the contents: * Directed Evolution of Ligninolytic Oxidoreductases: from Functional Expression to Stabilization and Beyond * New Trends in the In Situ Enzymatic Recycling of NAD(P)(H) Cofactors * Monooxygenase-Catalyzed Redox Cascade Biotransformations * Biocatalytic Redox Cascades Involving w-Transaminases * Multi-Enzyme Systems and Cascade Reactions Involving Cytochrome P450 Monooxygenases * Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates * Synergies of Chemistry and Biochemistry for the Production of Beta-Amino Acids * Racemizable Acyl Donors for Enzymatic Dynamic Kinetic Resolution * Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode * Perspectives on Multienzyme Process Technology * Nitrile Converting Enzymes Involved in Natural and Synthetic Cascade Reactions * Mining Genomes for Nitrilases * Key-Study on the Kinetic Aspects of the In-Situ NHase/AMase Cascade System of M. imperiale Resting Cells for Nitrile Bioconversion * Enzymatic Stereoselective Synthesis of Beta-Amino Acids * New Applications of Transketolase: Cascade Reactions for Assay Development * Aldolases as Catalyst for the Synthesis of Carbohydrates and Analogs * Enzymatic Generation of Sialoconjugate Diversity * Methyltransferases in Biocatalysis * Chemoenzymatic Multistep One-Pot Processes
About the Author
Dr. Sergio Riva is the Director of the Institute of the Chemistry of Molecular Recognition (ICRM) of the Italian National Council of Research (C.N.R.), where he has been working since 1984, and "Professore a Contratto"at the University of Modena and Reggio Emilia since 2000. He took his Laurea in Chemistry at Milano University in 1983 and his Diploma di Specialita in Organic Synthesis at Milano Politecnico in 1989. He spent one year (1987) at M.I.T. (Cambridge, USA) working with Prof. A. Klibanov. In 1993 he was awarded the Ciamician medal by the Organic Chemical Division of the Italian Chemical Society for his research activity in biocatalysis. His research activity is documented by more than 160 publications reporting on the isolation and characterization of different groups of enzymes (hydrolases, dehydrogenases, oxynitrilases, aldolases, laccases, glycosyltransferases), and on the use of these biocatalysts for the selective modification of natural compounds (steroids, alkaloids, terpenes, sugars and natural glycosides) and chiral synthons.
Wolf-Dieter Fessner obtained his PhD from the University of Freiburg, following which he carried out postdoctoral research at Harvard University with George Whitesides and at the University of Southern California with George Olah. Since 1998 he has been Full Professor of Organic Chemistry at the Technische Universitat Darmstadt. His primary research interest is focused on the development of practical methods for asymmetric synthesis at the interface between chemical and biological catalysis. A distinctive element in his research is the assembly and development of one-pot, multi-enzymatic reaction cascades for biocatalytic carbon-carbon bond formation and oligosaccharide synthesis, including the characterization and engineering of novel enzyme catalysts for a broader spectrum of substrate specificity
