The authors describe basic theoretical concepts of vibrational spectroscopy, address instrumental aspects and experimental procedures, and discuss experimental and theoretical methods for interpreting vibrational spectra. It is shown how vibrational spectroscopy provides information on general aspects of proteins, such as structure, dynamics, and protein folding. In addition, the authors use selected examples to demonstrate the application of Raman and IR spectroscopy to specific biological systems, such as metalloproteins, and photoreceptors. Throughout, references to extensive mathematical and physical aspects, involved biochemical features, and aspects of molecular biology are set in boxes for easier reading. Ideal for undergraduate as well as graduate students of biology, biochemistry, chemistry, and physics looking for a compact introduction to this field.
From the Contents: - Introduction - Introduction to infrared absorption and Raman spectroscopy - Instrumentation - Experimental Techniques - Proteins - Retinal proteins and photoinduced processes - Heme proteins - Non-heme metalloproteins
Wiley Tutorials in Biophysics is a series edited by A. Herrmann (Institute of Biology/Biophysics, Humboldt-University Berlin) and K.-P. Hofmann (Charite, Humboldt-University Berlin). Biophysics is the branch of physics focused on the study of biological systems. This series address the key issues within this rapidly growing field of research.
Friedrich Siebert is Professor for Biophysics at the University of Freiburg. He studied physics in Freiburg and Hamburg, receiving his PhD in solid-state physics. Since his diploma thesis he is working with different methods of vibrational spectroscopy. In 1972 he changed to biophysics, establishing the method of static and time-resolved infrared difference spectroscopy. Current research interests are photo-biological systems, membrane proteins and receptors, surface-enhanced techniques, time-resolved IR techniques.
Peter Hildebrandt received his PhD in chemistry from the Universitat Gottingen in 1985. After a post-doc stay in Princeton, he worked in research institutes in Gottingen, Mulheim, and Lisboa. Since 2003 he is Professor for Physical Chemistry and Biophysical Chemistry at the Technische Universitat Berlin. His research is dedicated to vibrational spectroscopy of biological systems, focussing on Raman spectroscopic techniques applied to redox proteins and photoreceptors.
About the Author
Friedrich Siebert is Professor for Biophysics at the University of Freiburg. He studied physics in Freiburg and Hamburg, receiving his PhD in solid-state physics. Since his diploma thesis he is working with different methods of vibrational spectroscopy. In 1972 he changed to biophysics, establishing the method of static and time-resolved infrared difference spectroscopy. Current research interests are photo-biological systems, membrane proteins and receptors, surface-enhanced techniques, time-resolved IR techniques.
Peter Hildebrandt received his PhD in chemistry from the Universität Göttingen in 1985. After a post-doc stay in Princeton, he worked in research institutes in Göttingen, Mülheim, and Lisboa. Since 2003 he is Professor for Physical Chemistry and Biophysical Chemistry at the Technische Universität Berlin. His research is dedicated to vibrational spectroscopy of biological systems, focussing on Raman spectroscopic techniques applied to redox proteins and photoreceptors.
