Quantitative calculations on the rates of enzyme-catalyzed reactions can help identify mechanisms of catalysis and calculate the speed of catalyzed reactions under various conditions. This book shows how to use mathematical applications such as Mathematica to conduct these calculations more easily and for more complicated mechanisms.

• Systematically describes the kinetics of an enzyme-catalyzed reaction using six steps, or six levels of understanding

• Provides the rate equations for a variety of types of enzyme-catalyzed reactions under various temperature, pH, and ionic strengths

• Requires a minimal number of velocity measurements in order to determine kinetic parameters for enzymatic reactions

• Shows step-by-step instructions for these calculations plus worked-out examples

• Provides a CD-ROM with the entire text of the book as well as programs you can use to estimate kinetic parameters from your own experimental data