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Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes, 2nd Edition

ISBN: 978-3-527-60985-7

February 2007

330 pages

Description

Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method, which has proven its effectiveness in the analysis of pharmaceuticals, food and the environment. This new edition of the practical guide to TLC includes a completely revised chapter on documentation, which now covers the use of digital cameras, while selected new sorbents and instruments are also introduced.

This edition retains the successful features of its predecessor:

  • Clear explanation of all steps in the analytical procedure, starting with the choice of a suitable TLC technique and finishing with data evaluation and documentation.
  • Special emphasis on the correct choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering precoated layers, solvents and developing chambers, including information on suppliers, among others.
  • Many practical hints for troubleshooting.
  • Detailed description of using TLC in compliance with GLP/GMP regulations, including the required documentation , allowing readers to easily compile their own standard operating procedures.
  • Numerous color illustrations.

From the reviews of the first edition:

"A clear, step-by-step practical guide to TLC, covering every stage of the process from plate selection and sample application to development and quantification. At every stage potential pitfalls are clearly signposted and each chapter contains a profusion of pracitcal tips. Even a seasoned TLC practitioner should find something of use or interest in this book which complements most existing texts."
The Analyst

About the Author

After working for many years for Heumann Pharma (now part of Pfizer), where she was in particular responsible for the training and education of lab technicians, Elke Hahn-Deinstrop is now a freelance consultant for thin-layer chromatography. She is author of numerous papers and frequent lecturer.