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Downstream Industrial Biotechnology: Recovery and Purification

ISBN: 978-1-118-61898-1

July 2013

872 pages

Description
DOWNSTREAM INDUSTRIAL BIOTECHNOLOGY

An affordable, easily accessible desk reference on biomanufacturing, focused on downstream recovery and purification

Advances in the fundamental knowledge surrounding biotechnology, novel materials, and advanced engineering approaches continue to be translated into bioprocesses that bring new products to market at a significantly faster pace than most other industries. Industrial scale biotechnology and new manufacturing methods are revolutionizing medicine, environmental monitoring and remediation, consumer products, food production, agriculture, and forestry, and continue to be a major area of research.

The downstream stage in industrial biotechnology refers to recovery, isolation, and purification of the microbial products from cell debris, processing medium and contaminating biomolecules from the upstream process into a finished product such as biopharmaceuticals and vaccines.

Downstream process design has the greatest impact on overall biomanufacturing cost because not only does the biochemistry of different products ( e.g., peptides, proteins, hormones, antibiotics, and complex antigens) dictate different methods for the isolation and purification of these products, but contaminating byproducts can also reduce overall process yield, and may have serious consequences on clinical safety and efficacy. Therefore downstream separation scientists and engineers are continually seeking to eliminate, or combine, unit operations to minimize the number of process steps in order to maximize product recovery at a specified concentration and purity.

Based on Wiley’s Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, this volume features fifty articles that provide information on down- stream recovery of cells and protein capture; process development and facility design; equipment; PAT in downstream processes; downstream cGMP operations; and regulatory compliance.

It covers:

  • Cell wall disruption and lysis
  • Cell recovery by centrifugation and filtration
  • Large-scale protein chromatography
  • Scale down of biopharmaceutical purification operations
  • Lipopolysaccharide removal
  • Porous media in biotechnology
  • Equipment used in industrial protein purification
  • Affinity chromatography
  • Antibody purification, monoclonal and polyclonal
  • Protein aggregation, precipitation and crystallization
  • Freeze-drying of biopharmaceuticals
  • Biopharmaceutical facility design and validation
  • Pharmaceutical bioburden testing
  • Regulatory requirements

Ideal for graduate and advanced undergraduate courses on biomanufacturing, biochemical engineering, biopharmaceutical facility design, biochemistry, industrial microbiology, gene expression technology, and cell culture technology, Downstream Industrial Biotechnology is also a highly recommended resource for industry professionals and libraries.

About the Author
Editor-in-Chief Dr. Michael C. Flickinger is the Associate Director for Academic Programs of the Golden LEAF Biomanufacturing Training and Education Center (BTEC) and a Professor of Microbiology and Chemical and Biomolecular Engineering at North Carolina State University, Raleigh. 

Dr. Flickinger was Director of the University of Minnesota's Institute for Advanced Studies in Biological Process Technology, which was later renamed the Biotechnology Institute ), and a Professor of Biochemistry, Molecular Biology and Biophysics at the University of Minnesota, St. Paul. Prior to coming to Minnesota Dr. Flickinger was Director of the Fermentation Program at the National Cancer Institute in Frederick, Maryland.

Dr. Flickinger received his academic training at the University of Wisconsin, Madison.  (B.S. (1973) in microbial biochemistry;  M.S. (1975) and Ph.D. (1977) in pharmaceutical biochemistry.)  He did his postdoctoral work at the Laboratory for Renewable Resources Engineering  in the School of Chemical Engineering, Purdue University. 

He is Editor-in-Chief of the Encyclopedia of Industrial Biotechnology (Wiley, 2010) and Co-Editor of the Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (Wiley, 1999).